CHAPTER 2    CHEMICAL LEVEL OF ORGANIZATION

Our bodies are made of chemicals and our life processes involve chemical reactions. A special name for chemistry that relates to living beings is biochemistry.

 

All living and nonliving things consist of matter.

Matter--anything that occupies space and has mass (weight).  Comes in 3 forms:

     Solid

     Liquid

     Gaseous

All forms of matter are composed of chemical elements. Elements are described as the building blocks of matter. There are 112 elements, 92 of which occur in nature. All matter is made up of either 1 pure element or a combination of elements. Definition of an element: a substance that cannot be split into simpler substances by ordinary chemical reactions.

 

Elements have names and also are represented by abbreviations called chemical symbols:

     Carbon     C

     Nitrogen   N

     Hydrogen   H             

     Calcium    Ca             If  2 letters are used, the first is a capital and the second is not

     Chlorine   Cl

     Sodium     Na

 

 

Our bodies contain 26 elements:     (See Table 2.1 P. 29)

     Oxygen

     Carbon                      These first 4 on the list make up 96% of the body

     Hydrogen

     Nitrogen

Lesser elements:

     Calcium                       Sulfur                 Magnesium

     Phosphorus                 Sodium               Iron

     Potassium                   Chlorine             

These 8 make up 3.8% of the body.

 

The remaining 14 are present only in tiny quantities (0.2% all together)  and are called trace elements.

 

 

Atoms--smallest units of matter having the characteristics of a particular element. An atom consists of even smaller subatomic particles. Three of these particles are of greatest importance in understanding chemical reactions. Two are found in a dense central core, the nucleus. The third kind travels (orbits) around the nucleus. Particles:

 

     1. Proton--positively charged and found within the nucleus. Have a mass of 1 dalton (a dalton is a very tiny unit used to measure mass of subatomic particles).

 

     2. Neutron--neutral charge and also within the nucleus--mass of 1 dalton.

(All except hydrogen have neutrons.)

     

     3. Electron--has a negative electrical charge and orbits around the nucleus. The mass is so tiny we will consider them weightless for A & P purposes.

 

IMPORTANT FACTS ABOUT ATOMS:

 

     1. The number of electrons and the number of protons in an atom is normally the same, giving a neutral charge

 

     2. The number of protons in the nucleus is what makes the atoms of one element different from another. For example, a hydrogen atom always contains 1 proton--if it had 2 it would be a helium atom. An oxygen atom always contains 8 protons--7 would make it nitrogen; 9 would make it fluorine. There is a chart called the periodic table that shows how many protons are always present in the atoms of each element (appendix B in your text).

 

     3. The number of protons is the atomic number of the element, so each element has its own atomic number from 1 – 112.

 

     4. Since both protons and neutrons have a mass of approx. 1 dalton each, the number of protons plus the number of neutrons equals the mass of the atom, which is called the mass number.

 

     5. Atoms of each element have their own characteristic number of neutrons

           Carbon   6 protons                     atomic number = 6

                          6 neutrons                   mass number = 12

 

           Sodium   11 protons                   atomic number = 11

                          12 neutrons                 mass number = 23

 

     6. Under unusual conditions, the number of neutrons in certain atoms of some elements may vary (remember protons MUST remain constant). Atoms having a variation in neutron number are called isotopes of the element. They behave the same as normal atoms in chemical reactions, but their mass has changed.

 

            Normal oxygen atom:   8 protons              atomic number = 8

                                               8 neutrons            mass number  = 16

Occasional atoms may have 9 or 10 neutrons, giving mass numbers of 17 or 18.     

 

     7. Although most isotopes are stable, certain isotopes of certain elements are radioactive because their nuclear structure seeks to change (decay) to a simpler and more stable form. As the change takes place, radiation (waves of energy with special properties) are emitted. If exposed to this energy, normal cells are damaged or destroyed by having their molecules broken apart. This is what makes atomic bombs so dangerous, but we also make use of radiation in medicine in the form of x-rays and by administering radioactive substances for special tests and treatments:

     Radioactive iodine to thyroid gland    Brain scans

     Thallium imaging of heart                  Cancer treatment

     Bone scans

 

 

More on the structure of atoms:

 

 

                                                                                      Shell #1   2 spaces

 

                                                                                             Shell #2   8 spaces

                                                    N

          Nucleus                             P                                       Shell #3  8 spaces  OR

                                                                                                             18 spaces

 

 

 

 

Electrons orbit around the nucleus. They were once believed to follow set paths called electron shells around the nucleus. We now know the motion is more random, but for A & P we will stick to the paths. (See Fig. 2-1 p. 30).

 

Each shell has space for a set number of electrons. Every space will not always be filled, but shell #1 will fill completely before any electrons go to shell #2; #2 will fill completely before #3 begins to fill.

 


The outermost shell that contains any electrons in a particular atom is known as the valence shell and the electrons in that shell may be called valence electrons.

 

For stability, atoms are always seeking to achieve a completely filled outer shell by giving up, gaining or sharing electrons with other atoms. This giving up, gaining or sharing of electrons results in chemical reactions.

 

 

 

 

                  OXYGEN                                         SODIUM

 

 

                          8 P                                                  11 P

                          8 N                                                  12 N

 

 

 

 

             8 electrons with 2                                 11 electrons with only

             empty spaces in                                    1 in outermost shell

             outermost shell                                     (7 empty spaces)

What if the outermost shell is naturally full? In that case (helium, neon, etc.) the element generally does not participate in chemical reactions and is said to be inert. Only those elements whose atoms have incompletely filled outermost shells tend to participate in chemical reactions.

 

                    For practice, see Fig. 2-2 P. 31

 

When 2 or more atoms combine, a molecule is formed. A molecule is held together by interaction among electrons.

 

A molecule may be formed by 2 atoms of the same element combining (H2, O2) or by 2 or more atoms of different elements (H2O, NaCl). If the molecules  contain 2 or more different elements, the resulting substance is called a compound. Since compounds are formed by chemical reactions they can also be broken down by chemical reactions (different from elements).

 

A free radical is an electrically charged atom or group of atoms with an unpaired electron in its outermost shell. Free radicals are formed by exposure to UV light, X-ryas, and normal metabolism. A common example is superoxide---an oxygen molecule that has gained 1 electron. Free radicals seek to either give up the unpaired electron or to take an electron from a normal cellular molecule. Free radicals are destructive and are currently believed to play a role in a number of diseases as well as in aging. Antioxidants appear to lessen the damage caused by free radicals.   Figure 2.3  P.  32

 

CHEMICAL BONDS

These are the forces of attraction that hold molecules together. They happen as atoms try to achieve completely filled outermost shells.  3 major types:

 

        1. IONIC BONDS--an atom normally has an equal number of protons and electrons and therefore a neutral charge. If it gains electrons it becomes negatively charged (anion); if it gives up electrons it becomes positively charged (cation). Table 2.2  P. 35 lists common ions in the body.

        Sodium tends to give up 1 electron and become positively charged

        Chlorine tends to gain 1 electron and become negatively charged

These charged atoms are called ions. When ions are in solution the solution will conduct an electric current, so ions are also called electrolytes.

 

As these positive and negative ions are formed they tend to be attracted to each other and this attraction is an ionic bond. (Fig. 2.4  P. 33)

 

In general:

     If the outer electron shell is less than half filled, the atom loses electrons and forms a positive ion (cation)

     If the outer electron shell is more than half filled, the atom gains electrons and forms a negative ion (anion)

     What if the outer shell is exactly half filled? Mostly these elements do not participate in ionic bonds but are more likely to enter covalent bonds. Hydrogen is one exception--it has 1 electron, so its outer shell (#1) is half filled. Even so, it has a tendency to give up its electron in some cases and become H+, a cation.

 

     2. COVALENT BONDS--these involve a sharing of electrons (1,2, or 3 pairs) and are the most common chemical bonds in the body. One example of this type of bond is the formation of hydrogen molecules, which also show the other way H can behave.

                                       (See Fig. 2.5  P. 34)

 

The electrons alternate--travel around first one nucleus and then the other, so each has a filled outermost shell half of the time. This can occur between 2 atoms of the same element or between atoms of different elements.

 

In a kind of shorthand, the sharing of one pair of electrons is represented:

     H    H

                                                                        

If 2 pairs of electrons are shared:                    H              H

 

     O    O                                                                 C

 

If 3 pairs are shared:                                        H            H

 

     N    N                                                              METHANE

 

Sometimes in a covalent bond, one atom attracts the shared electrons more strongly than the other. This is called a polar covalent bond. (If the attraction is equal the bond is a nonpolar covalent bond.) Water is formed by polar covalent bonds between H and O.        

              WATER (H2O)

 

           Electrons are more strongly attracted

        to the oxygen, to this end of the

          molecule becomes somewhat negative

 

 

                               O                          

                                                                       

                        H             H                        

                                                            

                   Electrons spend less time traveling around the H, so

                   this end becomes somewhat positive

 

 

3. HYDROGEN BONDS--2 other atoms (O or N) associate with a hydrogen atom. The H atom is covalently bonded to one O or N atom and is also attracted to the negative area of another O or N atom involved in a polar covalent bond.

 

 

 

 

 

 

 

 

 

 

Even though these bonds are relatively weak, they are still extremely important in biochemistry. Hydrogen bonds do not actually bind atoms into molecules and no electrons are shared or exchanged. However, they do act as bridges between molecules or different parts of the same molecule. In some large complex molecules a 3-dimensional shape is a necessary part of the structure and hydrogen bonds are what maintains this shape.

3 TYPES OF BONDS:

IONIC

COVALENT

HYDROGEN

GAIN OR LOSS OF ELECTRONS

SHARING OF ELECTRONS

NO ELECTRONS ARE GAINED, LOST OR SHARED, BUT AN ATTRACTION BETWEEN CERTAIN AREAS OF THE MOLECULE(S) INVOLVED HOLDS THOSE AREAS TOGETHER

MOSTLY IN INORGANIC

COMPOUNDS

MOSTLY IN ORGANIC COMPOUNDS (WATER IS AN EXCEPTION)

ORGANIC COMPOUNDS AND WATER

MEDIUM STRENGTH

 

STRONGEST TYPE

 

WEAK

 

 

CHEMICAL REACTIONS

 

Chemical reactions involve the making or breaking of bonds between atoms. They occur as atoms, ions and molecules collide. After a chemical reaction, the total number of atoms is the same, but new molecules with different properties have been formed.

 

Chemical reactions involve energy changes. Energy is the capacity to do work. Forms of energy:

   1. Potential energy is stored energy

   2. Kinetic energy is associated with matter in motion

   3. Chemical energy is stored in chemical bonds (a form of potential energy)

Energy can be converted from one form to another. In the process, heat is often released.

 

When atoms or molecules are joined by a chemical bond, energy is required to form the bond and the energy is stored in the bond. This type of reaction absorbs energy and is said to be endergonic. When the bond is broken, that energy is released. This reaction is described as exergonic.

 

Chemical reactions are described by chemical equations. A chemical equation looks like this:

      Mg  +  H2O                     à                     MgO  +  H2

The atoms, ions, or molecules on the                   The arrow                            The atoms, ions, or molecules on

left side of the arrow are what you                       represents                           the right side of the arrow are 

have BEFORE the reaction takes place.               the reaction.                         what you have AFTER the 

These are called the REACTANTS.                                                                    reaction takes place. These are

                                                                                                                        called the PRODUCTS. 

                                                                                                                                                                    

 

In a chemistry course, you will spend a lot of time balancing equations. We don’t worry much about this in here, but what it means is that to properly represent  a chemical  reaction, every atom shown on the left side of the arrow (the before side) must also appear in some form on the right side of the arrow ( the after side).

 

Atoms, ions, and molecules are continuously moving and colliding with each other. If the collision is forceful enough, an existing chemical bond may break or a new one may form. Activation energy is the minimum amount of energy required for a chemical reaction to occur. Two things make chemical reactions more likely:

   1. Concentration—increasing the concentration increases chances of a collision

   2. Temperature—speeds up movement

 

Catalysts speed up chemical reactions by lowering the activation energy required for the reaction to start. At the end of the reaction, the catalyst is unchanged. Catalysts are essential for chemical reactions in the body, since temperature, pressure and concentration are generally too low for reactions to occur at a speed that would sustain life. Raising these would kill the cells, so the solution is biological catalysts in the form of enzymes.

 

In all living things, large numbers of chemical reactions are constantly taking place. Metabolism is the sum of all chemical reactions occurring in a living organism. Types of chemical reactions:

 

     1. SYNTHESIS REACTIONS--joining of 2 or more ions, atoms or molecules to form new and larger molecules. In order to do this, new chemical bonds are formed and energy is absorbed (endergonic reaction). This phase of metabolism is called anabolism. In our bodies, an example is the linkage of amino acids to form proteins.

             A   +   B     à         AB            C   +   O2     à         CO2

                Reactants                   Product

 

2. DECOMPOSITION REACTIONS--bonds are broken--larger molecules are broken down into smaller molecules, atoms or ions. As bonds are broken, energy is released (exergonic reaction).

 

                AB     à       A   +   B

 

                CH4     à     C     +    2H2

 

This phase of metabolism is catabolism. An example in the body is digestion of food molecules. As bonds are broken, energy is released for use by cells.

 

     3. EXCHANGE REACTIONS--this type of reaction combines the first 2 types:

 

              AB     +    CD    à    AD     +    BC

 

Bonds forming molecule AB and bonds forming molecule CD are broken (decomposition). Then new bonds are formed (synthesis) and molecules AD and BC result. In the body buffer reactions are exchange reactions.

 

     4. REVERSIBLE REACTIONS--some reactions can go either way depending on conditions. Under one set of conditions:

                       A   +   B    à    AB

 

            Under another set of conditions:

                       AB   à   A   +   B

 

            All this can also be expressed as:

                     A   +  B         AB

                            

 

Conditions that might determine which direction the reaction will proceed include:

     A. Temperature

     B. pH

     C. Relative amount of reactants or products present

     D. Amount of energy available

     E. Presence or absence of catalysts (catalysts are atoms or molecules that can speed up the rate of a reaction without themselves being consumed or changed in the reaction). In reactions occurring in living cells, the catalysts are enzymes.

 

A great many biochemical reactions are reversible.

 

 

 

 

 

 

 

 

 

 

BIOCHEMISTRY—chemical reactions in living cells

Most of our chemicals are in the form of compounds. There are 2 types:

1. Inorganic

      Usually held together by ionic bonds

      Small molecules

      Usually LACK carbon

      Examples:

         Water

         CO2

         Salts

         Acids

         Bases

 

2. Organic

     Held together by covalent bonds (many)

     Large molecules

     ALWAYS have C ; almost always also have H

     Carbon atoms tend to form chains or rings

     Examples:

         Proteins

         Carbohydrates

         Lipids

 

INORGANIC COMPOUNDS

 

WATER

Water--most abundant substance of the body--around 55-60%; the only tissues low in water are bones and teeth. It is the most important inorganic compound in living things.

 

Terms to help explain the role of water in the body:

 

Mixture—combination of elements or compounds that are physically blended together but not held by chemical bonds. 3 types of mixtures:

   1. Solution--solvent + solute

           Solvent--liquid or gas in which other materials dissolve

           Solute--thing that dissolves

   2. Colloid—particles are larger and the mixture looks cloudy, but will not settle out

   3. Suspension--material mixes with medium but will settle out

 

Properties of water:

1. Water is an excellent solvent and suspending medium. It is the solvent in our body fluids. Many substances (but not all) will dissolve in water. This is mostly due to its polar covalent bonds.

 

            O     More negative

         H     H   More positive

NaCl dissolves in water because:

      O end is attracted to the Na+ portion

      H2 end is attracted to the Cl- portion

This pull takes the NaCl molecule apart (dissolves it).

 

Hydrophilic—substance will dissolve—solutes usually charged or contain polar covalent bonds

 

Hydrophobic—will not dissolve—usually contain nonpolar covalent bonds

 

As a solvent in the body, water:

       a.Dissolves and suspends many substances, allowing metabolic reactions to occur between dissolved substances

       b.Dissolves certain waste products so they can be removed from the body

 

2. Water participates in chemical reactions, both synthesis and decomposition.

3. Water absorbs and releases heat slowly--helps maintain homeostasis as surrounding temperature  changes

4. Water cools the body by evaporation of perspiration--water requires a large amount of heat to change from a liquid to a gas

5. Water serves as a lubricant--mucus, fluid in joints, fluid in ventral body cavity, food passing through digestive system

 

INORGANIC ACIDS, BASES AND SALTS

When molecules of these dissolve in water, they undergo ionization or dissociation---separate into + and - ions.

 

Acid—substance that when dissolved in water dissociates into 1 or more H+ ions and 1 or more anions (negative ions)

    HCl  à   H+  +  Cl-

Base—dissociates into 1 or more hydroxyl ions (OH-) and one or more cations (positive)

        NaOH  à   Na+   +   OH-

Salt—when dissolved in water dissociates into cations other than H+ and anions other than OH-

    NaCl   à   Na+   +   Cl-

 

Salts are formed when acids and bases react together:

     HCl   +   KOH  à   KCl   +   H2O

    Acid         Base       Salt

 

 

Salts are present in intracellular and extracellular fluid. This makes the fluids able to conduct electrical currents, so the ions of salts are sometimes called electrolytes. Ions of salts also provide many essential chemical elements, and solid forms of calcium salts give strength to bones and teeth.

 

ACID-BASE BALANCE